1352 Ectopic fibroblasts to modify skin identity

نویسندگان

چکیده

Skin identity is controlled by a combination of intrinsic features the epidermis and dermis as well crosstalk between two compartments. The modification skin has clinical potential, such conversion residual limb/stump (Non-volar) an amputee to pressure-responsive palmoplantar (Volar) in effort enhance prosthesis use minimize breakdown. Greater KRT9 expression, higher epidermal thickness, larger keratinocyte cytoplasmic size, longer collagen length are markers volar likely contribute resiliency. Given capacity fibroblasts modify differentiation, we hypothesized that might partially influence above features. First, demonstrated unique gene expression changes external pressure forces on vitro. We then confirmed induce bioprinted constructs. subsequently tested effects mismatched (i.e. ectopic) non-volar trial healthy volunteers. find increased measures after fibroblast injection maintained even 5 months. Total single cell RNA seq demonstrates ectopic have greatest effect ontology categories cornified envelope, keratinization, morphogenesis epithelium, with immuno-histologic pEGFR Notch pathway (RBPJ). This approach suggests novel therapeutic angle for any site-specific diseases.

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ژورنال

عنوان ژورنال: Journal of Investigative Dermatology

سال: 2023

ISSN: ['1523-1747', '0022-202X']

DOI: https://doi.org/10.1016/j.jid.2023.03.1368